Aspects of Tumour Targeting Preclinical Studies on Human Malignant Cells in vitro
نویسنده
چکیده
........................................................................................................ 8 POPULÄRVETENSKAPLIG SAMMANFATTNING ................................. 10 ABBREVIATIONS ........................................................................................... 13 FOREWORD ..................................................................................................... 15 INTRODUCTION ............................................................................................. 16 CANCER ............................................................................................................ 16 CONCEPTS OF TARGETING ................................................................................ 22 AIMS ................................................................................................................... 31 COMMENTS ON MATERIAL AND METHODS ........................................ 32 CELL CULTURE CONDITIONS (PAPER I-IV) ........................................................ 32 VISUAL INSPECTION (PAPER I-IV) .................................................................... 36 BORON UPTAKE STUDIES (PAPER I-III) ............................................................. 36 RAPID CELL FILTRATION (PAPER I-III) .............................................................. 37 WASHING PROCEDURE ...................................................................................... 38 INDUCTIVELY COUPLED PLASMA ATOMIC EMISSION SPECTROSCOPY (PAPER I-III) ......................................................................................................................... 39 3 H-THYMIDINE INCORPORATION STUDY (PAPER II) .......................................... 41 RADIOLABELLING (PAPER IV) .......................................................................... 41 CONJUGATION PROCEDURE (PAPER IV) ............................................................ 42 ACCUMULATION, RETENTION AND LOCALISATION OF RADIOLABELLED PDGFAA-DEXTRAN CONJUGATES (PAPER IV) ........................................................... 44 AUTORADIOGRAPHY ......................................................................................... 44 STATISTICS AND CALCULATIONS ...................................................................... 46
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